HFSP Career Development Award holder Florence Besse and colleagues
Friday 29th May 2015
How the transport of various molecules to neuronal processes (dendrites and axons) is regulated in vivo, in response to developmental signals, is currently largely unknown. Here, we describe a novel protocol enabling live-imaging of fluorescently-labeled molecules transported along axons in maturing Drosophila brains. This protocol can be combined with sophisticated genetic tools or drug treatments, and will be useful to researchers wishing to dynamically study the cellular mechanisms underlying...
HFSP Long-Term Fellow Nicola Aceto and colleagues
Tuesday 26th May 2015
Recently, breakthrough technological advances have made it possible, for the first time, to isolate and characterize extraordinarily rare circulating tumor cells (CTCs) from the blood of patients with cancer. Using both patient samples and mouse models, coupled with microfluidics technology and single cell resolution RNA sequencing, we found that CTC-clusters, held together by strong cell-cell junctions, represent metastatic precursors in the circulation of patients with cancer. Furthermore...
HFSP Career Development Award holder Marcelo Nollmann and colleagues
Friday 22nd May 2015
Rho is a ring-ATPase responsible for the general modulation of gene expression in Escherichia coli. We investigate the mechanism by which Rho translocates single-stranded RNA at the single-molecule level.
HFSP Long-Term Fellow Adrien Peyrache and Program Grant holder György Buzsáki and colleagues
Thursday 21st May 2015
The neuronal compass is activated in an organized manner during sleep, exactly as if animals were awake. This demonstrates that the system is hard-wired in such a way that it provides the navigation system with a reliable and unambiguous signal at all times.
HFSP Career Development Award holder Suvendra Bhattacharyya and colleagues
Tuesday 19th May 2015
Mature microRNAs (miRNAs) are stabilized in growth-retarded mammalian cells owing to increased sequestration with poly ribosomes (polysome) which results in increased levels of functionally inactive miRNPs. Polysomal arrest also leads to reduced export of these miRNAs via exosomes thereby restricting turnover of these regulatory molecules.
HFSP Career Development Award holder Danny Hsu and colleagues
Monday 18th May 2015
Polypeptide chains, like yarn and headset wires, can get entangled and knotted, but in a defined way. While such an idea was inconceivable for structural biologists two decades ago, recent structural genomic initiatives and developments of protein knot detection algorithms have helped identify hundreds of knotted proteins with different knotted topologies, ranging from a simple trefoil knot to a very complex Stevedore’s knot (Figure 1). Using MJ0366 from Methanocaldococcus jannaschii,...
HFSP Program Grant holders Philip Bevilacqua and Sarah Assmann and colleagues
Tuesday 12th May 2015
RNA folds into fascinating structures that govern almost every biological process. One distinctive structural motif, the G-quadruplex, is of particular interest given its potential relevance in regulation of biological processes. Our research describes a stable G-quadruplex structure within the 5’ UTR of Arabidopsis thaliana ATR mRNA that can inhibit translation, offering new insights into translational regulation in plants.
HFSP Program Grant holder Hang Lu and colleagues
Thursday 7th May 2015
The study of first larval stage Caenorhabditis elegans (soil nematode) is of particular interest for research on developmental biology. Traditionally it has been labor intensive to study them because of their small size and high motility. We have overcome this challenge by developing a miniaturized platform that uses nanoliter droplets of a reversible hydrogel to offer advanced manipulation of individual animals.
HFSP Program Grant holder Teva Vernoux and colleagues
Friday 1st May 2015
Auxin is the “conductor” of plant morphogenesis. Highly sensitive sensors and reporters now allow the dynamics of auxin distribution and auxin-induced transcription to be followed quantitatively in living tissues.
HFSP Long-Term Fellow Magor Lorincz and colleagues
Friday 17th April 2015
Active and inactive states (sometimes referred to as UP and DOWN states) represent a fundamental activity pattern of neocortical networks in a range of mammalian species including mice, rats, cats and humans. They typify the activity of the brain during both deep sleep and anesthesia and are also related to key aspects of sensory processing. Our research describes for the first time the mechanism that underlies the robust rhythmic initiation of UP states that is a fundamental characteristic of these...