HFSP Career Development Award holder Shay Ben-Aroya and colleagues
Friday 26th June 2015
Our study used the baker yeast Saccharomyces cerevisiae as a model system to reveal for the first time how the dysfunctional proteasome, which is associated with age-related pathologies and all the major chronic neurodegenerative disorders, is controlled by the protein quality control machinery.
HFSP Long-Term Fellow Stephan Preibisch and colleagues
Monday 22nd June 2015
The ability to track fast moving particles in 3D is limited in classic light microscopy as image stacks are acquired as a series of two-dimensional planes. Here, we reconstruct data from multifocal-microscopy that instantaneously captures entire 3D image stacks of live cells and show that β-actin mRNAs freely access the entire nucleus.
HFSP Cross-Disciplinary Fellow Ruben Portugues and colleagues
Thursday 18th June 2015
Animals use sensory evidence to decide what to do and when to do it. In fact, when presented with certain stimuli, it may take up to several seconds for an animal to react, even though action potentials, which govern the flow of information in the brain, last only a few milliseconds. Why?
HFSP Long-Term Fellow Andreas Mayer and colleagues
Tuesday 2nd June 2015
How genomes are transcribed by RNA polymerases in living cells is poorly defined. Here, we developed a genomic approach that visualizes RNA polymerase II transcription at single-nucleotide resolution in human cells. This approach reveals new general features of gene transcription and defines the transcriptional landscape of human cells.
HFSP Career Development Award holder Florence Besse and colleagues
Friday 29th May 2015
How the transport of various molecules to neuronal processes (dendrites and axons) is regulated in vivo, in response to developmental signals, is currently largely unknown. Here, we describe a novel protocol enabling live-imaging of fluorescently-labeled molecules transported along axons in maturing Drosophila brains. This protocol can be combined with sophisticated genetic tools or drug treatments, and will be useful to researchers wishing to dynamically study the cellular mechanisms underlying...
HFSP Long-Term Fellow Nicola Aceto and colleagues
Tuesday 26th May 2015
Recently, breakthrough technological advances have made it possible, for the first time, to isolate and characterize extraordinarily rare circulating tumor cells (CTCs) from the blood of patients with cancer. Using both patient samples and mouse models, coupled with microfluidics technology and single cell resolution RNA sequencing, we found that CTC-clusters, held together by strong cell-cell junctions, represent metastatic precursors in the circulation of patients with cancer. Furthermore...
HFSP Career Development Award holder Marcelo Nollmann and colleagues
Friday 22nd May 2015
Rho is a ring-ATPase responsible for the general modulation of gene expression in Escherichia coli. We investigate the mechanism by which Rho translocates single-stranded RNA at the single-molecule level.
HFSP Long-Term Fellow Adrien Peyrache and Program Grant holder György Buzsáki and colleagues
Thursday 21st May 2015
The neuronal compass is activated in an organized manner during sleep, exactly as if animals were awake. This demonstrates that the system is hard-wired in such a way that it provides the navigation system with a reliable and unambiguous signal at all times.
HFSP Career Development Award holder Suvendra Bhattacharyya and colleagues
Tuesday 19th May 2015
Mature microRNAs (miRNAs) are stabilized in growth-retarded mammalian cells owing to increased sequestration with poly ribosomes (polysome) which results in increased levels of functionally inactive miRNPs. Polysomal arrest also leads to reduced export of these miRNAs via exosomes thereby restricting turnover of these regulatory molecules.
HFSP Career Development Award holder Danny Hsu and colleagues
Monday 18th May 2015
Polypeptide chains, like yarn and headset wires, can get entangled and knotted, but in a defined way. While such an idea was inconceivable for structural biologists two decades ago, recent structural genomic initiatives and developments of protein knot detection algorithms have helped identify hundreds of knotted proteins with different knotted topologies, ranging from a simple trefoil knot to a very complex Stevedore’s knot (Figure 1). Using MJ0366 from Methanocaldococcus jannaschii,...