Frizzled2 pathway: applying the brakes on tumor metastasis

Metastasis is responsible for ~90% of cancer-associated mortality, yet progress has been slow in developing drugs that either specifically target metastasis or target cells with metastatic potential. Our study describes a new non-canonical Wnt pathway through Frizzled2 (Fzd2) that drives epithelial-mesenchymal transition (EMT) and tumor metastasis.

HFSP Long-Term Fellow Taran Gujral and colleagues
authored on Thu, 13 November 2014

The epithelial-mesenchymal transition (EMT) is a reversible process in which epithelial cells adopt mesenchymal properties including altered morphology, cellular architecture and migratory capacity. Several growth factors, including TGFβ, Wnt, EGF, FGF, and HGF have been shown to trigger EMT in both embryonic development and normal and transformed cell lines. However, mechanistic understanding of how specific growth factors induce EMT is still lacking. Uncovering the signaling pathways by which growth factors regulate EMT could have broad biological significance and potentially guide the development of new therapies directed at cancer metastasis.

In this study, we describe a new non-canonical Wnt pathway through Frizzled2 receptor that drives EMT, cellular migration, and tumor metastasis. We show that among the large set of known Frizzled receptors and Wnt ligands, Fzd2 and Wnt5a/Wnt5b are selectively overexpressed in metastatic cancer cell lines and in high-grade human tumors, with high expression correlating with markers of EMT. Reducing receptor expression by RNAi or blocking receptor activity with anti-Fzd2 antibodies reduced Wnt5-mediated cell migration in vitro and inhibited tumor growth and metastasis in a mouse xenograft model.

Using a variety of systematic genetic and chemical tools, we dissected Fzd2 signaling in a panel of liver, breast, and lung cancer cell lines and showed that Wnt5a-Fzd2 binding leads to Stat3 activation, which in turn drives a pro-EMT transcriptional program. Using the newly developed statistical method (Gujral et al., PNAS), we identified the cytosolic tyrosine kinase Fyn as the key mediator of Fzd2-driven Stat3 phosphorylation. Fyn is recruited to a newly identified site of Fzd2 tyrosine phosphorylation and mediates activation of Stat3.  Furthermore, a gene signature regulated by this pathway predicts metastasis and overall survival in patients.

Finally, our findings are particularly significant because we show that the oncogenic activity of Fzd2 can be inhibited by an antibody –representing a new therapeutic strategy for treating metastatic disease.

Reference

1. A Noncanonical Frizzled2 Pathway Regulates Epithelial-Mesenchymal Transition and Metastasis..Gujral, T. S., Chan, M., Peshkin, L., Sorger, P.K., Kirschner, M.W., and G. MacBeath. Cell 2014, Volume 159 , Issue 4 , 844 – 856.

Other references

2. Exploiting polypharmacology for drug target deconvolution. Gujral, T.S., Peshkin, L., and Kirschner, M.W. PNAS 2014 111 (13) 5048-5053; doi:10.1073/pnas.1403080111

Link to Cell article

Pubmed link (Ref 2)