It has long been thought that memories are stored in the brain by strengthening the connections between neurons. However, recent discoveries suggest that molecules inside cells, especially RNA, might also be involved in storing and transferring memories. Our project explores this idea by studying a free-swimming organism called Spirostomum ambiguum and immune cells known as macrophages. These single cells are capable of learning from their experiences, much like more complex organisms. For example, they can \"train\" themselves to respond more effectively to challenges they’ve faced before. A key tool in our research is Live-seq, a cutting-edge technology that allows us to study the activity of individual cells over time without killing them. Traditional methods for studying cells typically destroy the cell during analysis, limiting what we can learn. Live-seq, however, enables us to track gene activity in living cells, making it possible to see how a cell’s behavior and gene expression change as it \"learns.\" We are particularly interested in how cells can exhibit associative memory—a form of learning similar to how Pavlov’s dogs learned to associate a bell with food. In cells, this could mean \"remembering\" a previous stimulus and preparing a stronger response the next time. Additionally, we will explore the possibility of implanting synthetic or \"false\" memories into cells by transferring RNA from one cell to another, potentially demonstrating that RNA can carry memory-specific information. This research could change how we understand memory in all living organisms and might even lead to new medical applications, such as improved treatments for immune-related diseases.